| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Alkalihalobacillus alcalophilus | ATGCTCCAAGGGTCGTAGC | CCGAATAAAGCTGCGGTGG | 59.48 | 59.28 | 159 | 158 |
100.00%
|
100.00%
|
| Primer 2 | Alkalihalobacillus alcalophilus | GCTTTCAAGAGCTGCCACC | ATCGGGTACTGTGCCATCC | 59.42 | 59.17 | 238 | 155 |
100.00%
|
100.00%
|
| Primer 3 | Alkalihalobacillus alcalophilus | ACGGCATGGGCAATTTGC | GGCCAAACCGGTTTCTTGC | 59.73 | 60.30 | 167 | 150 |
100.00%
|
100.00%
|
| Primer 4 | Alkalihalobacillus alcalophilus | TGCGGATGGATTCCCTGAT | CAGGGCTATTGTCGGGAGT | 58.46 | 58.79 | 167 | 141 |
100.00%
|
100.00%
|
| Primer 5 | Alkalihalobacillus alcalophilus | ACAGACGTGATATGACCGAGC | TCATTCAGGCCACCTCGTG | 59.93 | 59.70 | 172 | 137 |
100.00%
|
100.00%
|
| Primer 6 | Alkalihalobacillus alcalophilus | TCCCTGACTCTGATGACCCA | CAGGGCTATTGTCGGGAGT | 59.59 | 58.79 | 156 | 135 |
100.00%
|
100.00%
|
| Primer 7 | Alkalihalobacillus alcalophilus | AGGAGAGTTTTGGCAGGCTG | TTCATGCCATCCCCACCAC | 60.25 | 60.00 | 258 | 133 |
100.00%
|
100.00%
|
| Primer 8 | Alkalihalobacillus alcalophilus | GGAGAGTTTTGGCAGGCTG | TTCATGCCATCCCCACCAC | 58.74 | 60.00 | 257 | 133 |
100.00%
|
100.00%
|
| Primer 9 | Alkalihalobacillus alcalophilus | GCACGTTGCTACCACAATGG | CCCCATTGAACCACTTGTGC | 60.11 | 59.68 | 261 | 132 |
100.00%
|
100.00%
|
| Primer 10 | Alkalihalobacillus alcalophilus | ATGCGGATGGATTCCCTGA | CAGGGCTATTGTCGGGAGT | 58.46 | 58.79 | 168 | 132 |
100.00%
|
100.00%
|